goat anti mouse 412 lyve1 antibody  (R&D Systems)

Journal: bioRxiv

Article Title: Mapping the spatial architecture of glioblastoma from core to edge delineates niche-specific tumor cell states and intercellular interactions

doi: 10.1101/2025.04.04.647096

Figure Lengend Snippet: (A) Top 6 cell types enriched within 20 micron radius of CD8+ GZMK+ T cells. Enrichment score was calculated by dividing the frequency of a target cell type within a given radius of a root state (CD8+GZMK+ T cells) by the frequency of the target cell type within the sample. Each dot represents a patient sample. (B) Distance to the nearest target myeloid cell of each CD8 +. GZMK + T cell separated by localization within a vascular or non-vascular niche. Each dot represents a single CD8 +. GZMK + T cell, with all CD8 +. GZMK + T cells across all samples visualized. (C) Distances from averaged on a pers sample basis. Each dot represents an individual sample. Significance was calculated using the Wilcoxon matched-pairs signed rank test. (D) Frequency of CD8 +. GZMK + T cells in the vascular niche given proximity to the target myeloid cell (either within 30 μm or further than 30 μm). (E) Frequency of CD8+GZMK+ T cells within 30 μm of target myeloid cell given presence of T cell in vascular or non-vascular niche. (F) Correlation of GZMK and LYVE1 expression based upon localization in vascular or non-vascular niche. Data derived from IVY-Gap. (G) Confocal imaging of immunofluorescence staining of human GBM tumor sample.

Article Snippet: Primary antibodies (diluted in blocking buffer) were used as follows: goat anti-LYVE1 antibody 1:30 (AF2089, RND Systems), rabbit anti-CD163 1:500 (93498, Cell Signaling Technology).

Techniques: Expressing, Derivative Assay, Imaging, Immunofluorescence, Staining